WebApr 11, 2024 · The cells on the coverslips were fixed with 4% paraformaldehyde for 20 min. After washing twice with PBS, the cells were treated with BODIPY 493/503 (1 μg/ml) for 20 min and DAPI (5 μg/ml) for 2 min. Images were acquired using a fluorescence microscope. Tumor growth and metastasis assay in nude mice WebCells also can be fixed and permeabilized after staining. LipidSpot™ stains show minimal background staining of cellular membranes or other organelles, unlike traditional dyes like Nile Red. Find the Right Stain for …
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WebSep 25, 2024 · Here we describe a method to stain LDs, which can be applied to previously fixed mouse oocytes and embryos. This method is based on fluorescent dyes, Nile red … WebApr 7, 2024 · Safranin-O staining. Cells were fixed in 70% ethanol and incubated in 0.1% safranin-O for 5 min at room temperature and serially rinsed with double distilled water, 70% ethanol, and 100% ethanol. ... chondrogenic (C), and osteogenic (D) differentiation was assessed by oil Bodipy staining of neutral lipids, safranin-O staining of cartilage, and ... remote work technology trends
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WebAug 17, 2016 · ADRP Staining Perilipin2/ADRP is a protein ubiquitously and specifically associated with cytoplasmic lipid bodies in various types of cells. 1. Prepare fixed cell samples as described above in ‘Cell sample preparation’. 2. Permeabilize the cells by placing the sample carrier into 0.1% Triton X-100 in Ca 2+ /Mg 2+-free HBSS for 10 min … WebMay 18, 2024 · It is rather used for live staining, but it works well on fixed cells/tissues. ... After testing several dyes, CellTrace™ BODIPY® TR Methyl Ester from ThermoFisher worked absolutely great (red ... Webthe culture dish, rinse with HBSS, and add prewarmed staining solution. Incubate the cells for approximately 15–30 minutes at 37˚C. Replace the staining solution with fresh probe-free medium and view the cells using a fluorescence microscope. If the stained cells are to be fixed, refer to the fixation steps below for the appropriate dye. remote work wage and hour issues