Bzr1 chip-seq
WebFeb 27, 2024 · ChIP-sequencing analysis, and yeast one-hybrid and dual-luciferase assays demonstrated that the protein binds to the promoters of AP2/EREBP genes (Zm00001d010676 and Zm00001d032077) and inhibits their transcription. cDNA library screening showed that ZmBES1/BZR1-5 interacts with casein kinase II subunit β4 … WebJan 26, 2024 · In this chapter, the RNA-seq and ChIP-seq protocols to identify BR target genes are described and an overview of the methods for data analyses is presented as …
Bzr1 chip-seq
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WebChromatin immunoprecipitation (ChIP) is a powerful method for studying TF-DNA interactions in vivo. To identify all binding sites of a TF in the genome, the DNA obtained in ChIP experiments needs to be analyzed by hybridization to genome-tiling microarrays (ChIP-chip) or by next-generation sequencing (ChIP-seq). WebTo generate comparable data sets, we performed BZR1 ChIP-Seq analysis using dark-grown seedlings (Figure 1—source data 1B), as used in the ARF6 and PIF4 ChIP-Seq (Oh et al., 2012). Interestingly, large portions of ARF6 binding targets overlapped with either tar-gets of BZR1 (51%) or PIF4 (71%) or both (42%) (Figure 1C). The common targets ...
Web总 RNA 提取和 qRT-PCR 分析\RNA-Seq. Western Blot\重组蛋白纯化和 EMSA\染色质免疫沉淀 (ChIP)-qPCR. 双荧光素酶测定\酵母单杂交试验 ... ( C ) WT 植物、 bzr1和hy5突变体侧芽中BRC1的转录水平。 ... WebThe function of BZR1 on organ size is well characterized in Arabidopsis, but poorly understood in maize (Zea mays). ... ChIP and RNA-seq analysis showed ZmBZR1 can directly bind to the promoter region of organ size related genes, Germination Repression and Cell Expansion receptor-like kinase (GRACE) and KIP-RELATED PROTEIN6 …
WebApr 14, 2024 · The PIF4–BZR1 complex tends to directly activate genes involved in cell elongation and responses to auxin and GA but indirectly represses genes encoding photosynthetic proteins. ... The integration of … WebAug 4, 2024 · Sources for RNA-seq data related to BZR1 are in legend Table 2 ... PIF4, BZR1, HY5, and MYC2 from ChIP-seq experiments . as available at PlantPan3.0 sho w 34 TFs with binding to the .
WebMar 11, 2005 · Lane S is the G + A chemical sequencing reaction. Footprint regions are marked by vertical lines and corresponding sequence. (D) RBSS for optimal BZR1 binding site. Shown on the top is the alignment of all identified sequences, with the matching sequences marked in red. ... we performed chromatin immunoprecipitation (ChIP) …
WebFeb 9, 2024 · Transcriptome profile of BZR1-responsive genes during seed germination via RNA-seq. To explore other BZR1-responsive targets involved in regulating rice germination, ... (2008) with minor modifications. In brief, young panicles of BZR1-OX. ChIP-qPCR was performed as described previously (Tong et al., 2014). t/hbfia 0024—2021WebAug 4, 2024 · Comparing PIF-, BZR1-, HY5-, and MYC-Related ChIP-seq and RNA-seq Data Although DNA ChIP-seq data indicate a central role for PIF4 by binding to the … thb filterWebJan 18, 2024 · The genome-wide ChIP-Seq analysis revealed that ARF6 shares a vast number of genomic targets (around 50%) with BZR1 and the light/temperature-regulated transcription factor PIF4 by CHIP-Seq analyses (Oh et al., 2014). BZR1 and PIF4 interact with ARF6 and activate shared target genes by binding to shared target genes … thbfixedWebApr 7, 2012 · variation, as we did for the BZR1 ChIP-chip experiment (17). 1. ... Although several tools for the analysis of ChIP-seq data have been published recently, there is a growing demand, in particular ... thb fitnessWebOct 7, 2024 · The bri-t-like phenotype of bzr-h suggested that BR signaling was completely shut off in the bzr-h mutant. To obtain evidence to support this conclusion, we treated 2 … thb fisioWebApr 3, 2024 · Because BZR1 and its closest homolog BES1 act as key transcription factors downstream of BIN2 in the BR-signaling pathway (He et al., 2002, Yin et al., 2002), we examined the freezing tolerance of their dominant active mutants, bzr1-1D and bes1-D.The bzr1-1D and bes1-D mutants showed increased freezing tolerance compared with the … thbfix 6mWebMar 21, 2024 · FZR1 (Fizzy And Cell Division Cycle 20 Related 1) is a Protein Coding gene. Diseases associated with FZR1 include Developmental And Epileptic Encephalopathy … thb filter regulator dryer