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WebSep 22, 2024 · The recombinant pBD2 showed high bactericidal activity. E. coli were cracked and dead after incubation with 20 μg/mL pBD2 for 1 h and 4 h, the debris was observed, and the bactericidal effect was more obvious at 4 h than those at 1 h ( Figure 2 ). Figure 1 Analysis of purified pBD2 by SDS-PAGE. WebThe recombinant pBD2 was expressed after induction by IPTG and purified by His tag affinity column with 95% purity. The recombinant pBD2 exhibited antimicrobial activity against both Gram-positive S. aureus and Gram-negative E. coli including the multi-resistant E. … WebDec 29, 2015 · Porcine β-defensin 2 (pBD2) is an antimicrobial peptide in pigs that plays an important role in the immune system by preventing bacterial invasion. To produce an anti-pBD2 antibody, which is not commercially available, we expressed and purified a soluble, his-tagged version of pBD2 (his-pBD2). hippo swimming speed