Enliten atp assay promega madison wi
WebENLITEN® ATP Assay System 100 assays ₩ 377,000 Your price: Log in The ENLITEN® ATP Assay System can be used to measure ATP levels for the indirect detection of biocontamination on food processing surfaces, in cosmetics and beverages or to assay for enzymes that degrade ATP and to quantitate ATP in biological fluids. WebMay 9, 2024 · After being treated for different time points (0.5, 1, 1.5, and 2 h), the supernatant of cells was analyzed using the ENLITEN ATP luciferase assay kit (Promega, Madison, WI, USA). The level of extracellular ATP chemiluminescence was measured using the Wallac VICTOR 3 Multilabel plate reader (PerkinElmer, Shelton, CT, USA).
Enliten atp assay promega madison wi
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WebNov 25, 2015 · Purified or immobilized Luc was mixed with luciferin, Mg 2+, K + and ATP as indicated in individual experiments and activity was monitored by means of light emission as detected by a luminometer (GloMax, Promega, Madison, WI), where the luminescence signal was integrated for 3 seconds every minute for up to 50 minutes, as indicated per … WebThe Enliten ATP Assay System is a bioluminescence detection kit which is designed to measure down to 0.1 femtomoles of ATP. As shown in Figure 5, TopCount achieved this detec- tion limit, 0.1 femtomoles, and had a linear range of 10-12to 10-15 moles.
WebDec 15, 2024 · Supernatant was collected and analyzed using an ENLITEN ATP assay (Promega, Madison, WI, USA) and HMGB1 ELISA kit II (Shino-Test, Kanagawa, Japan). Western blotting. Cells were seeded in a 100-mm dish at a density of 1 × 10 6 cells/dish 24 h before viral infection. Murine and human PDAC cells were infected with OBP-301, Ad … Web4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TB268 · Revised 11/15 …
WebEnter the email address you signed up with and we'll email you a reset link. Webthe ENLITEN® ATP Assay INTRODUCTION The GloMax® 96 Microplate Luminometer combined with the ENLITEN® ATP Assay System Bioluminescence Detection Kit …
WebMar 18, 2016 · The supernatants of RH2-infected SCCVII cells and uninfected control cells were collected; and cell debris was removed by centrifugation at 6200 r.p.m. for 5 min. Secreted extracellular ATP in the ...
WebThe CellTiter-Glo® 2.0 Cell Viability Assay exhibits the same high performance as the classic CellTiter-Glo® Assay, making it suitable for use from benchtop scale to high-throughput screening. High sensitivity and broad linearity. Luminescence measured with the CellTiter-Glo® 2.0 Assay is proportional to the number of viable Jurkat cells in ... diablo 3 rabid strike explainedWebAug 30, 2024 · We used an ATP assay kit (Enliten, Promega, Madison, WI, USA) to estimate the ATP concentration in the supernatant by measuring in the luminescence channel of a Cytation 5 plate reader (BioTek Instruments, Inc., Winooski, VT, USA). The results are expressed in nanomolar ATP per milligram of proteins. diablo 3 queen of the succubusWeb2 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TB306 · Revised 5/09 www.promega.com 1. Description The CytoTox-ONE™ Homogeneous Membrane Integrity Assay(a) is a homogeneous, fluorometric method for estimating the number of … diablo 3 rare scoundrel tokenWebVarious pain therapies have been developed on the basis of the gate control theory of pain, which postulates that nonpainful sensory inputs mediated by large-diameter afferent fibers (Aβ-fibers) ca... cinema therapy coralineWebNov 5, 2024 · Supernatant was collected and analyzed with ENLITEN ATP assay (Promega, Madison, WI, USA) and HMGB1 ELISA kit II (Shino-Test, Kanagawa, Japan). Western blot analysis K7M2 and NHOS cells, seeded in a 100-mm dish at a density of 2 × 10 5 cells/dish, were infected with OBP-502 at MOIs of 0, 10, 50, 100, 500, and 1000 … diablo 3 ps4 wizard setsWebNext, 10 mL of each sample were dispensed in a blank 96-well plate and 90 mL of luciferin/luciferase reagent were added (Enliten ATP Assay System; Promega, … diablo 3 rainbow worldWebq Promega ENLITEN ® ATP Assay System Bioluminescence Detection Kit for ATP (P/N FF2000) containing one vial rLuciferase/Luciferin (rL/L) Reagent, 12 mL Reconstitution Buffer, one vial ATP Standard (1x10-7 M), 25 mL ATP-Free Water B ... Madison, WI 53711 USA. Title: 997_9307_1_0.doc cinema therapy gollum