Goldenstar rt6 cdna synthesis mix
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Goldenstar rt6 cdna synthesis mix
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WebFeb 17, 2024 · Total RNA was extracted from normal skin and HS tissues of rat tails using TriPure Reagent (BIOFIT, Chengdu, China). Next, cDNA was synthesised from RNA using the Goldenstar RT6 cDNA Synthesis Kit Ver 2 (Tsingke Biotech Co., Beijing, China). RT-qPCR was performed in a 2xT5 Fast qPCR Mix (SYBR Green I; Tsingke Biotech Co.). WebGoldenstar® RT6. cDNA Synthesis Mix (gDNA remover and Rnasin selected)/50℃反应，使用方便，反应快速，热稳定性强，含DNase和Rnase抑制剂/反转录系列试剂/擎科生 …
WebFeb 11, 2024 · Subsequently, 0.8 µg of total RNA from each sample was used for cDNA synthesis using Goldenstar™ RT6 cDNA Synthesis Kit Ver.2 (Tsingke Biotech Co., Ltd., Beijing, China) according to the manufacturer’s instructions. ... CA, USA) using Hieff UNICON ® Universal Blue qPCR SYBR Green Master Mix (Yeasen Biotech Co., Ltd., … WebJun 1, 2024 · The Trelief™ RNAprep FastPure Tissue & Cell, Goldenstar™ RT6 cDNA Synthesis, and SYBR Green I (2 ×T5 Fast qPCR Mix) kits were all purchased from TsingKe Biotech Co., Ltd., Beijing, China. 2.5. Crayfish culture for challenge experiments. The A. hydrophila from our laboratory. We incubated it in a common agar medium at 28 °C for …
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WebIf you are new to cDNA synthesis or experience researcher wanting to optimize your protocol, consider these five critical steps to help you ensure your cDNA synthesis results in highest efficiency. Learn reverse transcription basics Find reverse transcriptases See also five steps for fast RT-PCR. Step 1 Prepare. sample. Step 2 Remove. genomic DNA.
Web丁香通商铺北京擎科生物科技股份有限公司为您提供实验室仪器 / 设备、试剂、技术服务、细胞库 / 细胞培养、抗体、elisa 试剂盒、耗材等分类产品5件，全方位满足您的科研实验采 … ford 360 fe headsWebOct 14, 2024 · Then, the cDNA was synthesized following the protocol of Evo M-MLV RT Premix for qPCR (Accurate Biotechnology, Hunan, China) and Goldenstar™ RT6 cDNA Synthesis Kit (TsingKe, China). Quantitative real-time PCR was carried out using Master qPCR Mix (TsingKe, China) on a CFX96 Real-Time PCR system (Bio-Rad, CA, USA). elk population in yellowstone before wolvesWebDec 1, 2024 · The reverse transcription experiment was carried out by Goldenstar™ RT6 cDNA Synthesis Kit Ver2 (Tsingke, Beijing, China). The cDNA product was diluted as a qPCR template and amplified with 2×T5 Fast qPCR Mix(SYBR Green I, Tsingke, Beijing, China). A Real-time PCR System (BIOER) was used to amplify. ford 360 fe long blockWebTotal RNA was extracted from the cells using Trizol reagent (Ambion, Life technologies), and 1 μg of RNA was reverse-transcribed using the Goldenstar RT6 cDNA Synthesis Mix (Takara, Kyoto, Japan) according to the manufacturer’s instructions. elkport iowa campgroundWebApr 26, 2024 · And there were higher expression levels of lignin synthesis- and lignifications-related genes in the GhWAT123-silenced plants. ... following the manufacturer’s protocol. First-strand cDNA was synthesised from 3 μg of total RNA using the Goldenstar RT6 cDNA Synthesis kit Ver. 2 (Tsingke, Beijing, China) to analyse the … ford 360 flywheelWebSep 6, 2024 · The first strand of cDNA was synthesized according to the instructions for the Goldenstar TM RT6 cDNA synthesis Mix (TIANGEN, Beijing, China). Subsequently, qRT-PCR was performed using the SYBR PrimeScript RT-PCR Kit (TIANGEN, Beijing, China) with SYBR Green dye. The maize actin gene ZmActin was used as the internal control. elk post officeWeb丁香通为您找到150条一次Pcr扩增有多少基因厂家的工商注册年份、员工人数、年营业额、信用记录、主营产品、相关一次Pcr扩增有多少基因产品的供求信息、交易记录等企业详情。丁香通为买家提供用户服务，诚信保障等服务，批发采购一次Pcr扩增有多少基因，上丁香通。 elk post office hours