WebDescription. The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit featuring: Fast—short reaction time (typically 30–60 min.); Convenient—easy workflow with few pipetting steps (2 tubes); Reliable—reverse transcription of both abundant and limited targets; Flexible—optimized two-step protocol, enabling multiple PCR reactions from a … Web3 de out. de 2016 · I am using the high capacity cdna reverse transcription kit by applied biosystems catalog no 4374967 to make the cDNA from my input RNA of 500ng, my reaction size for RT is 20uL as recommended in ...
iScript Advanced cDNA Synthesis Kit for RT-qPCR
WebHá 3 horas · RNA (500 ng) was reverse transcribed using MultiScribe Reverse Transcriptase (Applied Biosystems) according to instructions in the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ... WebGuidelines for Using the Kit Recommendations for RNA For optimal performance of the High-Capacity cDNA Archive Kit, Applied Biosystems recommends using RNA with the following characteristics: ♦ Greater than 60 µ L of sample ♦ Between 0.002 and 0.2 µ g/ µ L in concentration of RNA ♦ Less than 0.5% of genomic DNA by weight ♦ ctv marketplace investigations
High-Capacity cDNA Reverse Transcription Kit
WebThe High Capacity RNA-to-cDNA Master Mix optimizes cDNA synthesis for high-throughput, real-time gene expression analysis. • Single tube, complete first-strand cDNA synthesis master mix reduces experimental variability and streamlines workflow • 5X concentration provides robust results even with dilute RNA samples and rare or abundant ... Web12 de jan. de 2024 · There is a maximum limit to any kit like Takara 1st strand prime script cDNA kit which has a max 5ug capacity. Also, we must dilute the cDNA because qPCR is a sensitive reaction and so a high cDNA ... WebArticle Snippet: Total RNA was extracted from the transfected cells 72 h post-transfection using the NucleoSpin RNA Plus (Clontech), and 250 ng of purified RNA was used for cDNA synthesis in 20ul of total reaction using High-Capacity RNA-cDNA kit (ThermoFisher). cDNA was diluted 1:20 and 3 μL of cDNA was used for qPCR using SYBR Green Real … easiest chicken soup recipe