Mitotracker gfp-lc3
WebThe marriage between immunology and cytometry is one of the most stable and productive in the recent history of science. A rapid search in PubMed shows that, as of March 2024, using "flow cytometry immunology" as a search term yields more than 60,000 articles, the first of which, interestingly, is not about lymphocytes. Web6 jun. 2024 · Finally, the relationship between Parkin translocation to mitochondria and selective mitophagy was checked by observing merged images of Parkin, GFP-LC3 and …
Mitotracker gfp-lc3
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Web4 jul. 2024 · MTG (MitoTracker green)可以示踪线粒体. LTR (LysoTracker red)可以示踪溶酶体. 无自噬时，GFP-LC3融合蛋白弥散在胞浆中；自噬形成时，GFP-LC3融合蛋白转位 … Web单丹磺酰戊二胺（monodansylcadaverine，MDC）（大连宝生物工程有限公司），细胞计数试剂盒（cell counting kit-8，CCK-8）（美国Invitrogen 公司），Mitotraker Red 探针（美国Invitrogen 公司），一抗兔抗人β-actin、微管相关蛋白1 轻链3（microtubule-associated protein 1 light chain 3，LC3）、CCAAT 增强子结合蛋白 α（CCAAT/enhancer ...
Web在过表达三种类型的tau后，用MitoTracker Red染料标记线粒体，发现过表达TauWT和TauKR，线粒体长度明显增加，而过表达TauKQ后，线粒体长度减少。 与Vec组相比，TauWT在小鼠海马CA1区的过表达增加了Mfn1、Mfn2和Opa1的mRNA和蛋白水平，过表达TauKR上调了Mfn1和Opa1水平，而TauKQ明显减少了这些融合蛋白的表达水平。 Web26 jul. 2024 · ATG7 KO BT549 cells transfected with PA-GFP and co-labeled with MitoTracker-red, with frames taken before and after 405 nM light. Scale bar represents …
WebMitoTracker is taken up electrophoretically by mitochondria, dependent on the mitochondrial membrane potential. Unlike TMRM, it covalently binds to mitochondrial proteins and thus remains in... Web14 apr. 2024 · In addition, the mCherry signal was more punctate in CBFB_KO cells than in WT cells, indicating that most mCherry-EGFP-LC3B in CBFB_KO cells formed …
WebBriefly, we employed a SOX2-enhancer-GFP-reporter system to isolate cancer cells with high SOX2 transcriptional activity by FACS sorting. ... MitoTracker Deep Red (MTDR) ... degradation of Cav-1, we see the upregulation of a number of well-established autophagy/mitophagy markers, namely LC3, ATG16L, BNIP3, BNIP3L, HIF-1α and NFκB.
Web10 apr. 2014 · Autophagy plays an important role in tumorigenesis. Mitochondrion-associated protein LRPPRC interacts with MAP1S that interacts with LC3 and bridges … scientific name for tiger sharkWebExpression of GFP-LC3 in ARPE-19 cells at a cell density of 1.0 × 10 6 cells was performed by transfection with 2.5 μg pEGFP-LC3 plasmid ... Live-cell fluorescence microscopy with the MitoTracker Green FM dye to determine changes in mitochondrial morphology in cells after incubation with HQ or H 2 O 2 for 2 h. (B) ... scientific name for ticksWeb2 feb. 2015 · You can see Bnip3L, which is one of the molecules involved in mitophagy. Bnip3L present an interaction domain with LC3, and then labelled in autophagosomes … scientific name for timeWebIntroduction to Adenovirus-LC3 production service for Autophagy Flux Detection. Autophagy is a highly regulated homeostatic degradative process where cells destroy their own … praxair flemingtonWeb29 dec. 2009 · In each of these 3 conditions, Parkin-YFP translocation to MitoTracker Deep Red-labeled mitochondria was observed in cells that consistently had the lowest ΔΨ m as assessed using tetramethyl rhodamine methyl ester (TMRM) fluorescence ( Fig. 1 E ). scientific name for timberwolfWeb27 mei 2024 · The Aggresome Detection Kit was acquired from Abcam (Cambridge, MA, USA). MitoTracker Deep Red, the ROS 2′,7′-dichlorodihydrofluorescein diacetate … scientific name for third eyeWebConfocal microscopy and the mitochondrial dye Mitotracker green were used to obtain three-dimensional images of the mitochondrial network in cardiomyocytes and L6 skeletal muscle cells in ... Both small aggregates and aggresomes colocalized with autophagy markers such as GFP-LC3 and Rab24. Glucose deprivation stimulates reactive oxygen ... scientific name for thyme herb