Nrf1 chip
Web[13]. Indeed, NRF1 binding elements have been identified in genes involved in cellular growth, metabolic energy produc-tion, protein translation and DNA replication/repair [13]. ChIP-on-chip analysis of human T98G glioblastoma-derived cells showed that NRF1 binds to ~691 genes encoding pro-teins regulating cellular growth, DNA replication, DNA re- WebChIP was performed using K562 cells, the Diagenode monoclonal antibody against NRF1 (Cat. No. C15200013) and optimized PCR primer sets for qPCR. ChIP was performed …
Nrf1 chip
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Web3 okt. 2024 · Relative mRNA levels of Nrf1 (a) and Acaa2 (b) were measured after Nrf1 siRNA treatment. Cells were subjected to ChIP using an anti-NRF1 antibody or IgG … WebImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody (ab34682) Purified anti-NRF1 antibody (ab34682) was used at 5µg/ml to detect …
WebNRF1 (UniProt/Swiss-Prot entry Q16656) is a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes … Web3 okt. 2024 · Quantification of cDNA or ChIP-DNA samples was conducted via standard quantitative PCR (qPCR) methods using SYBR Green (Thermo Fisher Scientific). The following genes were tested: Acaa2, Gabpa, Mef2a, Nrf1 and Slc25a20 for rat cDNA; Acaa2, Cpt1a, Elovl6, Fads2, Mki67, Scd2, Slc25a20 and Top2a for mouse cDNA and …
Webusing the approach consisting of a systematic integration of ChIP DNA-seq, RNA-Microarray, NRF1 protein-DNA motif binding, signal pathway analysis, and Bayesian machine learning. Our findings show that a high percentage of known HER2+ breast cancer susceptibility genes, including EGFR, IGFR, and E2F1, are under transcriptional control … Web22 jan. 2014 · It is important to note that since we used an antibody that recognizes both Nrf1 isoforms, our ChIP data does not discriminate between the binding of either p65-Nrf1 or p120-Nrf1. In addition, our chip assay data demonstrating Nrf1 differentially binding at the ARE in the presence of hormone correlates with our IP studies that showed …
Web20 aug. 2024 · To identify network-based pathways involved in the susceptibility to the ER/PR −ve HER2 +ve breast cancer subtype, the NRF1 transcriptional regulatory genome-wide landscape was analyzed using the approach consisting of a systematic integration of ChIP DNA-seq, RNA-Microarray, NRF1 protein-DNA motif binding, signal pathway …
Web4 mei 2024 · Moreover, the transcription factor, Nuclear Respiratory Factor 1 (NRF1) exhibits a novel and integral role in this aspect of the neurodevelopmental process, being … bollywood dance class descriptionWebChIP - Anti-NRF1 antibody [EPR5554(N)] - BSA and Azide free (ab221792) Chromatin was prepared from NIH/3T3 treated with MG-132(2uM 16h) cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes. glynt alpha setting lotion 200 mlWeb21 aug. 2024 · Moreover, it has been reported that NRF-1 is a transcriptional activator of glutamate receptor subunit genes under conditions of depolarizing stimulation in neurons ( Dhar and Wong-Riley, 2009) suggesting that in addition to its role in cellular metabolism, via regulation of the mitochondrial genome, NRF-1 coordinates activities in the nucleus to … bollywood dance classes bay areaWeb4 nov. 2013 · NRF1 which activates transcription of nuclear-encoded mitochondrial proteins plays an integrative role in nuclear-mitochondrial interactions. 3 ChIP-Seq showed a … bollywood dance classes dcWebChIP-sequencing, also known as ChIP-seq, is a method used to analyze protein interactions with DNA.ChIP-seq combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. It can be used to map global binding sites precisely for any protein of interest. Previously, … bollywood dance classes cambridgeWebHere we have used two transcription regulatory proteins: nuclear respiratory factor 1 (NRF1) and inhibitor of differentiation protein 3 (ID3) for ChIP-Seq and RNA-Seq data as … glyn tarell community councilWebRabbit monoclonal [EPR5554(N)] to NRF1 - ChIP Grade. Application: ChIC/CUT&RUN-seq, ChIP, ChIP-seq, Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB. Reactivity: Mouse, Rat, Human. Conjugate: Unconjugated. Recombinant 10 µl Trial Size. Anti-SUZ12 antibody [EPR5234(N)] - ChIP Grade (ab175187) bollywood dance classes chicago