Organotypic hippocampal slices
Witryna5 mar 2013 · In this sense, the organotypic hippocampal slice culture (OHSC), if generated from adult brain, would be a promising ex vivo tool for various kinds of …
Organotypic hippocampal slices
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WitrynaAlign the hippocampus perpendicular to the chopper blade. Slices (250–400 µm) thick are rapidly cut (2/s) by the tissue chopper. Note: The thickness of the slices will depend on the age of the animal. The older the animal, the thinner should be the slice, with a compromise around 300 µm. 3. WitrynaAbstract. This protocol details a method to immunostain organotypic slice cultures from mouse hippocampus. The cultures are based on the interface method, which does not require special equipment, is easy to execute and yields slice cultures that can be imaged repeatedly, from the time of isolation at postnatal day 6–9 up to 6 months in …
Witryna30 lip 2015 · Organotypic hippocampal slice cultures (OHSCs) have been used as a powerful ex vivo model for decades. They have been used successfully in studies of … WitrynaThe organotypic hippocampal slice culture provides rapid assessment of the neuroprotective potential of novel compounds for the clinical management of SE. Importantly, this assay models the glutatmate-induced excitotoxicity found in SE (1, 2), thus is a predictive in vitro screen to identify potentially clinically-relevant compounds.
Witryna1 wrz 2005 · Organotypic Hippocampal Slice Cultures Current Drug Targets - CNS & Neurological Disorders, 2005, Vol. 4, No. 4 447 groups have extended the MEAs even further, recording from up to 64 ... Witryna21 mar 2024 · This paper examines the effects of blast-induced traumatic brain injury (bTBI) on long-term potentiation (LTP) and microglial …
WitrynaAbstract. Slices of developing brain tissue can be grown for several weeks as so-called organotypic slice cultures. Here we summarize and review studies using …
Witryna18 sty 2024 · Organotypic hippocampal slice culture (OHSC) Rat organotypic hippocampal-entorhinal cortical slice cultures were prepared as described previously [48, 49].Briefly, neonates rat pups at postnatal day 7 (P7) were decapitated, brain removed and hippocampal-entorhinal complex dissected in Gey’s buffer (Sigma … pershing global clearing servicesWitryna(A) Phase-contrast image of an organotypic hippocampal slice 3 weeks after in vitro maintenance. Scale bar, 70 µm. (B,C) The time course of changes in the passive membrane properties of CA1... stalin cannibal island documentaryWitrynaPreparation of Rat Organotypic Hippocampal Slice Cultures Using the Membrane-Interface Method Timothy W. Church & Matthew G. Gold, 2024, Springer Protocols. Preparation of Organotypic Slice Cultures for the Study of Glutamate Receptor Function Andres Barria, 2024, Springer Protocols. In Vitro Oxygen-Glucose Deprivation to … pershing general hospital clinicWitrynaNational Center for Biotechnology Information stalin bust ebayWitrynaAn increasing body of data has shown that matrix metalloproteinase-9 (MMP-9), an extracellularly acting, Zn2+-dependent endopeptidase, is important not only for pathologies of the central nervous system but also for neuronal plasticity. Here, we use three independent experimental models to show that enzymatic activity of MMP-9 … stalin buchWitrynaOrganotypic brain slice cultures and organoids; Ex-vivo brain preparation (e.g., eye-brain from turtles) Learn More About MaxOne. ... Multiple single-unit long-term tracking on organotypic hippocampal slices using high-density microelectrode arrays Journal Article. Frontiers in Neuroscience, 10 , pp. 1-16, 2016, ... stalin central committee membersWitryna30 paź 2024 · During and after organotypic culture (a) Bird's eye view of hippocampal slices inside Millipore semipermeable membrane insert. (b) Lentiviral injection using a fine gauge needle into the CA1 ... pershing general hospital nv