WebApr 9, 2024 · Sign up. See new Tweets WebOvalbumin (OVA) has traditionally been used to induce mouse allergic asthma models (2-5). Mice are intraperitoneally immunized with OVA adsorbed to an aluminum hydroxide …
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WebThe inner shell of the chestnut (Castanea crenata) contains various polyphenols, which exert beneficial biological effects. Hence, we assessed the anti-inflammatory efficacy of a chestnut inner shell extract (CIE) in ovalbumin (OVA)-induced allergic asthma. We intraperitoneally injected 20 μg of OVA with 2 mg of aluminum hydroxide on days 0 … Aqueous organic dust extract (ODE) was collected and prepared as previously described [22]. Briefly, settled surface dust samples (~ 3 ft off ground) from swine confinement animal feeding operations (~ 500–700 animals) were collected and 1 g was placed into sterile Hank’s Balanced Salt Solution (10 ml; … See more Male C57BL/6 mice (6–8 wk. old) were purchased from The Jackson Laboratory (Bar Harbor, ME) for consistency with prior agriculture exposure … See more In separate studies, bronchoalveolar lavage (BAL) fluid was collected using 3 × 1 ml PBS. Total cell numbers from pooled lavages were … See more Based upon previous work determining optimal time point to determine ODE-induced inflammatory responses [7], 3 h following the one-time treatment with intranasal inhalation … See more Cell-free BAL fluid was evaluated for cytokines and chemokines by ELISA. Levels of mediators previously implicated in mediating ODE-induced airway inflammation include TNF-α, IL-6, and the murine neutrophil … See more bob\u0027s burgers and brew happy hour
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WebNational Center for Biotechnology Information WebIn the present study, we investigated the protective effects of SA on allergic asthma in a murine model of ovalbumin (OVA)-induced asthma. Methods: BALB/c mice were … WebOvalbumin-Induced Asthma Model. OVA is the main protein found in egg white, which is not intrinsically immunogenic and therefore needs to be injected systemically in the presence … clitheroe mill hotel