Peasy-blunt载体序列
WebMay 31, 2024 · 分别利用T7引物与M13引物对pEASY质粒和两个pTOPO质粒所连接的片段进行PCR验证，产物进行琼脂糖凝胶电泳后如图1-B所示，pTOPO-ppPig、pTOPO-kpPig、pEASY-Pig重组质粒所连片段（泳道1、2、3），3个泳道的条带均在大于15 000 bp处有明显条带，与预期相符。 WebpEASY-Blunt clonning vector 载体说明书WORD版. LacZfragment: bases 1-548. M13 reverse priming site: bases 205-221. Multiple cloning site: bases 234-358. T7 promoter/priming site: bases 364-381. M13 Forward (-20) priming site: bases 389-404. 2319, 2692. AcuI (4) ctgaag.
Peasy-blunt载体序列
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WebOct 22, 2016 · pEASY-Blunt clonning vector 载体说明书WORD版.doc 22页. pEASY-Blunt clonning vector 载体说明书WORD版.doc. 22页. 内容提供方 ： me54545. 大小 ： 419 KB. 字数 ： 约2.76万字. 发布时间 ： 2016-10-22发布于河南. WebApr 13, 2024 · For probe labelling, specific 500 bp sequences of target genes were amplified from the complementary DNA (cDNA) of Wm82 with primers (Supplementary Data 9) and cloned to pEASY-Blunt cloning vector ...
WebAug 3, 2016 · pEASY- T1 Cloning Kits在3’ 端提供一个悬垂的T 碱基，这样的结构使PCR片段可以高效的和载体进行连接。. 牛痘拓扑异构酶I型可以与DNA双链 的特定位置结合并切割特异位点5’- (C/T)CCTT-3’, 与3‘T的磷酸基团性成共价键，并切断其中 一条DNA链，使DNA解链。. …
WebManual Vector Information MSDS COA. Inquire Now Buy Now Add to Cart Free Sample Avaliable. Product Details. pEASY® -Blunt Cloning Kit is designed for cloning and … WebAug 7, 2013 · 产品说明pEASY-T1CloningKits适用于TA克隆.-快速克隆基因，仅需要5分钟反应时间。. 对照插入片段克隆效率可达95%以上。. 包含LacZ基因，在含有IPTG，X-gal的平板培养基上，可进行蓝白斑筛选。. 工作原理pEASY-T1CloningKits以线性的形式提供:-3′端悬垂胸腺嘧啶 (T)可用于TA ...
WebpEASY® -Blunt E1 Expression Vector is constructed from pET vector, it utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient …
WebFigure Legend Snippet: Kinetic properties of recombinant PiGSTd1. (A) Purification of recombinant pEasy-Blunt-E1-PiGSTd1. Induced: the crude extracts from the bacterial pellets with 1mM isopropyl β-D-1-thiogalactopyranoside (IPTG) induction. 1–4: samples eluted with binding buffers containing 50, 100, 150, and 200mM imidazole, respectively. dr thayer vanderbilt plasticsWebApr 11, 2024 · The PCR product was inserted into the pEASY-Blunt Simple Cloning vector (TransGen Biotech, Beijing, China) and then transformed into DH5α for sequencing. MUSCLE software was employed for multisequence alignments 100, and a phylogenetic tree was constructed with MEGA X using the ML method 111. Internal branching support was … coltec industries incWeb接下来就要将目的片段连到中间载体pENTR3C上，将pENTR3C和pEASY-Blunt－目的片段这两个质粒用BamHI和EcoRI做双酶切，回收2.5K的载体片段和1.5K的目的片段，做连接，目的片段和载体片段的摩尔比为5：1，16度过夜连接，用的是TaKaRa的T4连接酶，已经连了三次 … coltec industries inc websiteWebpEasyBlunt用的这种T载体分好几种，有的是不带“blunt”的，有的是带blunt的，同时还有带多克隆位点还没有多克隆位点的。 用于克隆，但是它的连接片段须得是用保真酶（比 … dr thayer tonerWebpEASY ®-Blunt （3929 bp） LacZα fragment: bases 1-548 Multiple cloning site: bases 234-358 M13 reverse priming site: bases 205-221 T7 promoter priming site: bases 362-381 … coltec specialty productsWebAug 7, 2013 · 产品说明pEASY-T1CloningKits适用于TA克隆.-快速克隆基因，仅需要5分钟反应时间。. 对照插入片段克隆效率可达95%以上。. 包含LacZ基因，在含有IPTG，X-gal的平 … coltec toolpEASY-Blunt Simple (linearized) Cloning vector for blunt-ended PCR products, with blue-white selection, kanamycin and ampicillin resistance markers, and a T7 promoter but no multiple cloning site. Sequence Author : TransBionovo (TransGen Biotech) dr. thayer westfield ma