2024 Plp-creert2

Plp-creert2

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August undefined, 2024

WebbThis is reflected by the sequential expression of For activation of the CreERT2 protein, tamoxifen (Sigma T5648) was dissolved in 1:10 ethanol:sunflower oil at 10 mg/ml. Tamoxifen (1 mg) was transcription factors associated with lineage specification. injected intraperitoneally into the pregnant mother on 1 day for Plp- Neurogenin 2 (Neurog2), a … Webb11 mars 2024 · To rule out that the pathological profiles in Cmtm5 mutant mice are the consequence of subtle developmental defects, we used the Plp CreERT2 driver line …

Transgenic Mice for a Tamoxifen-Induced, Conditional Expression …

Webb基因别名： jp,Plp,msd,rsh,DM20,jimpy; ... *使用本品系发表的文献需注明：Plp1-P2A-Rox-creERT2-Rox mice（Strain NO.T053842）were purchased from GemPharmatech … speed of ultrasound in soft tissue

Fried Laboratory Karolinska Institutet - ki

Webb7 sep. 2024 · a Relative mRNA levels for Ccl2 and Csf1 normalized to the housekeeping gene rpl32 from ganglia isolated from the muscularis of the small intestine from naïve wild-type mice and 1.5 h, 3 h and 24 h... Webb一、品系介绍：. Cre-ERT2小鼠是一类含有雌激素受体（estrogen receptor，ER）的配体结合区突变体（ERT）与Cre重组酶的融合蛋白表达的小鼠。. Cre-ERT2在无Tamoxifen诱 … http://eprints.gla.ac.uk/256854/1/256854.pdf speed of trust trust cards

Inducible site‐specific recombination in myelinating cells ...

Tamoxifen诱导Cre-ERT2小鼠 cre小鼠品系介绍南模生物

WebbDNA was analyzed by PCR to identify PLP-CreERT2-positivemice(Leoneetal.,2003)and to distinguish erbB2 wild-type and lacZ alleles (Britschetal.,1998).Primersusedfordetecting PLP-CreERT2 alleles were as follows: 5 -CAC TCTGTGCTTGGTAACATGG,and5 -TGG GAT CCG CCG CAT AAC C (94°C for 2 min, 35timesat94°Cfor45s,53°Cfor30s,and72°C WebbThe targeting of reporter expression to inner phalangeal and border cells was increased when Plp-CreER T2 was paired with the CAG-eGFP reporter; however, the total number … speed of underground trainsWebb10 juli 2014 · Although the Plp-creERT2 allele has been shown to have variable activity in vivo (Doerflinger et al., 2003), we observed about 85% of Olig2+ cells expressed a … speed of ultraviolet light

"WebbPlp Creert2 Proteolipid Protein Mice 121, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - … " - Plp-creert2

Plp-creert2

Webb19 juni 2024 · Transgenic mice and experimental design. Myrf fl/fl mice [], which have LoxP inserted around exon 8 of Myrf, were crossed with PDGFRα-CreERT2 mice [].The Cre … WebbR26YFP (Laranjeira et al. 2011) and PLP-CreERT2/R26YFP mice were used for genetic cell lineage tracing of peripheral glia (Leone et al. 2003). R26Confetti mice were received …

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Webb南模生物. Language: 中文 . English; 日本語; 한국어; 账户设置; 退出 Webb22 mars 2024 · Through tdT expression in PLP-CreERT2 x tdT mice, PLP +-glial cells could be detected around the enteric neurons (Figure 4F), even better distinguished by the …

Webb14 juni 2024 · Abstract. NG2 (nerve/glia antigen-2) is a type I transmembrane glycoprotein and also known as chondroitin sulfate proteoglycan 4. In the parenchyma of the central … Webb22 jan. 2024 · The Cre cassette (CreERT2-mCherry) is 2.8 kb in size and single-strand DNA is gel-purified and used for microinjection. Table 1 lists all the validated gRNAs for each of the CRISPR donor constructs. Validation was done in rat embryos by microinjection of the gRNA with Cas9 protein.

Webb18 maj 2012 · CreERT2 has high affinity for the 4-hydroxytamoxifen (4-OHT) but not for the endogenous estradiol. After its exposure to the specific inducer, CreERT2 is translocated into the nucleus and mediates the recombination of floxed genes. Webb14 jan. 2024 · Proteolipid protein (PLP) is the most abundant integral membrane protein in central nervous system (CNS) myelin. Expression of the Plp -gene in oligodendrocytes is …

Webb27 nov. 2024 · In a PLP-CreERT2 pNF model, we have previously demonstrated that Schwann cells are susceptible to pNF development when Nf1 is ablated with tamoxifen …

Webb1 apr. 2003 · PLP-CreERT2 mediates recombination with high efficiency. To judge the recombination efficiency of PLP-CreERT2, 8-week-old PLP-CreERT2 R26R double … speed of usb 3.0WebbThe Plp-CreERT2;QkLoxP/LoxP (Qk-Plp-iCKO) mice and littermate controls were injected with tamoxifen at postnatal day 7 and day 8 and their brains were collected at postnatal … speed of universal expansionWebb将CreERT2-P2A插入到小鼠Cspg4基因起始密码子处。应用领域：Cre工具鼠，血管周细胞和胶质细胞相关品系 Cspg4-Flox 品系名： C57BL/6JSmoc- Cspg4em (flox)/Smoc 品系状态：在研目录号：NM-CKO-230168 Cspg4-KO 品系名： C57BL/6JSmoc- Cspg4em2Smoc 品系状态：胚胎冻存目录号：NM-KO-200141 你也可能感兴趣 Tamoxifen诱导Cre-ERT2 … speed of vcat vs appendWebb2 sep. 2024 · 128 CreERT2 in myelinating cells under the control of the Plp promotor (Leone et al., 2003) 129 we gained control mice (Mbpfl/fl*PlpCreERT2wt) and inducible … speed of v-22 ospreyWebb20 aug. 2024 · 为探究前庭扁平上皮的形成机制，研究团队利用前期已建立的前庭扁平上皮的建模方法，采用vGlut3-iCreERT2:Rosa26tdTomato、Glast … speed of usain boltWebbCover illustration: Lineage traced PLP-CreERT2/R26YFP dental mesenchymal stem cells and odontoblasts (orange) in the adult mouse incisor tooth. Immunohistochemistry … speed of usb c portWebbPlp-CreERT2;QKLoxP/LoxP (Qk-KO) mice and control mice were injected with tamoxifen at 8-week, and brains were collected 4 days after tamoxifen injection to isolate … speed of us fighter jets