Psh65 plasmid
WebBackground: Adherence to the surface of the host cell is the precondition for T. vaginalis parasitism and pathogenicity, causing urogenital infection. The AP65 of T. vaginalis … WebThe present invention discloses a strain of bacteria producing DHA and/or EPA, six gene fragments in the bacterial genome, and uses thereof. The strain is Schizoochytrium limacinum HS01, which has the accession number of CGMCC No. 13746 at China General Microbiological Culture Collection Center. The six gene fragments are composed of gene …
Psh65 plasmid
Did you know?
WebSep 13, 2024 · Transform pMM296 (pSH65 Carter and Delneri, 2010) into the strain of interest, plate on YPD, and replica plate onto YPD + 10 μg/mL Phleomycin the next day. The manufacturer's instructions are found here: Invivogen Product Information, Phleomycin. Inoculate Phleomycin-resistant colonies in YP+raffinose and let it grow overnight to … WebDownload pMCSG65.dna file Download Plasmid Open in SnapGene SnapGene SnapGene is the easiest way to plan, visualize and document your everyday molecular biology …
WebPlasmid: pSH. Shuttle expression vector utilizing a chimeric promoter derived from the HTLV-I LTR and the HIV-1 LTR. Supports high levels of expression in human and mouse … WebJan 26, 2013 · The pSH65 plasmid contained the sequences of the Cre recombinase gene, GAL1 promoter and TEF1 promoter of Saccharomyces cerevisiae, CYC1 terminator, Ble r and Amp r resistance genes.
WebPlasmid Files. Download over 2,700 carefully annotated plasmid and sequence files including commonly used cloning vectors from all major suppliers. Explore Over 2.7k … WebConstruction of plasmid pCU563 carrying a heterologous selection-marker gene flanked by loxP sites. A DNA fragment containing 34-bp loxP, the 0.45-kb PGKpromoter ( pr), and the …
WebApr 10, 2012 · To remove the hygromycin B resistance marker gene hphNT1, IMI058 and IMI059 were transformed with pSH65 and—after marker removal via the Cre/loxP system …
WebJul 1, 2013 · Loss of pSH65 was achieved by growing cells in liquid YPD medium at 30 °C for 2 days. 2.3. Determination of extracellular inulinase activity Recombinants were grown in 50 mL of YPI medium (2% peptone, 1% yeast extract and 2% inulin [w/v], pH 5.5) at 30 °C with shaking at 200 rpm. rnav missed approach pointWebOct 8, 2013 · pSH65 (P30122) deletion marker Reference: Güldener U, Heinisch J, Köhler GJ, Voss D, Hegemann JH. A second set of loxP marker cassettes for Cre-mediated multiple … rna viral sheddingWebOct 25, 2024 · To obtain the engineered Saccharomyces cerevisiae for high-yield endoglucanase, endoglucanase gene was cloned from Clostridium cellulovorans, with a total length of 1 996 bp, encoding 440 amino... snake and eagle shadow movieWebSelection Ampicillin, delta-ura3 Source Yeast, Escherichia coli Species Bacterial Q&A Customer Reviews Related Products For research use only. Not intended for any clinical use. Quick Inquiry Name Service & Products Interested * pSH65 (OVT2571) RESET USA SUITE 115, 17 Ramsey Road, Shirley, NY 11967, USA Germany Industriepark Höchst, Gebäude … snake and eveWebDear colleagues, friends, can everyone exchange plasmids?Our laboratory now needs two plasmids: PUG6, PSH65.There must be PFA6A -KANMX4.Our laboratory now has … snake and earringWebthe plasmid pUG6 as a template (Gueldener et al. 2002). Saccharomyces cerevisiae cells were trans- ... containing 300 lg G418/ml. The G418-resistant KanMX gene was excised with Cre recom-binase and expressed from the pSH65 plasmid (Gueldener et al. 2002). Heterogeneous expression of JJJ1 in BYDJJJ1 was performed by cloning the JJJ1 ORF … snake alley criterium 2022WebApr 11, 2016 · The G418-resistant KanMX gene was excised with Cre recombinase and expressed from the pSH65 plasmid (Gueldener et al. 2002 ). Heterogeneous expression of JJJ1 in BYΔJJJ1 was performed by cloning the JJJ1 ORF into the EcoR I and Kpn I sites of the pYES2 plasmid (Invitrogen, Carlsbad, CA, USA). snake ancestors