Roche rapid ligation kit
WebJun 1, 2006 · getting a bit frustrated with a triple ligation that doesn't seem to work (i.e. very few colonies, none with insert, very low background = religated vector) I was wondering if anybody has any advice concerning the issue: ... has anyone compared the Rapid ligation kit from Roche with the regular T4 ligase from NEB? Any comment appreciated ...
Roche rapid ligation kit
Did you know?
WebThermo Scientific Rapid DNA Ligation Kit enables fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.The kit contains T4 DNA ligase and a specially … WebROCHE Rapid DNA Dephos + Ligation Kit 160 Rxns Code: 4898125001 D2-231 Application The Rapid DNA Dephos & Ligation Kit minimizes the time needed to perform diverse applications, including: Cloning of fragments into either plasmid or phage vector... read more ADD £476.00 EACH List Price OVERVIEW Application
http://www.rpdata.caltech.edu/courses/aph162/2006/Protocols/RocheRapidDNALigation.pdf WebNote: The following protocol is for rapid ligation of cohesive ends. For rapid ligation of blunt ends, use T4 DNA Ligase, Cat no. 15224-041, which has a concentration of 5 U/µl. This higher concentration is required for rapid ligation of blunt ends. A molar ratio of 3:1 insert:vector is re commended for the rapid ligation of DNA
WebRapid DNA Dephos & Ligation Kit ROCHE/RDLIG-RO - sufficient for 40 reactions (04898117001), sufficient for 160 reactions (04898125001), optimum reaction temp. 37 … WebThe Fast-Link kits efficiently and rapidly ligate sticky end DNA in 5 minutes and blunt ended DNA in 15 minutes. Compare this item ROCHE Rapid DNA Ligation Kit MilliporeSigma …& Ligation Kit contains all the reagents of the Rapid DNA Ligation Kit plus our new rAPid Alkaline Phosphatase.
WebROCHE Rapid Alkaline Phosphatase 1000 Units Code: 4898133001 D2-231 Application Use rAPid Alkaline Phosphatase in numerous applications, including: Removal of 5′-phosphoryl groups from nucleic acidsPreparation of templates for 5′-end labeling... read more ADD £99.60 EACH List Price OVERVIEW Application
WebSep 21, 2005 · We use the Roche Rapid DNA Ligation kit that claims 5 min at RT (though I tend to let it go 15-20 min for good measure). I have also used regular T4 ligase and buffer and ligated O/N at 16oC. startech.com 4 port pci express usb 3.0 cardWebApplication Use T4 DNA Ligase to ligate DNA fragments with blunt or overlapping ends. Properties CAS Number: 9015-85-4 Enzyme activities: T4 DNA Ligase catalyzes the … peter thomson canadaWebManufacturer Roche Applied Science - a member of the Roche Group Be the first to review this product Upgrade from the Rapid DNA Ligation Kit and choose the new Rapid DNA … peter thomson net worthhttp://www.protocol-online.org/biology-forums/posts/16892.html peter thomson facebookWeb† add 10 l t4 dna ligation buffer ( vial 1) to the reaction vial. to spin the vial of canvax t4 dna ligase for a few seconds before pipetting the enzyme. heat inactivate at 65° c for 10 minutes. the kit contains t4 dna ligase and a specially- formulated 5x rapid ligation buffer optimized for fast and efficient dna ligation. peter thomson nhWebRapid DNA Dephos & Ligation Kit ROCHE/RDLIG-RO - sufficient for 40 reactions (04898117001), sufficient for 160 reactions (04898125001), optimum reaction temp. 37 °C Synonym: cloning of dna fragments; dna ligase; ligases Product Type: Chemical Site-directed mutagenesis by overlap extension PCR. peter thomsenWebROCHE Rapid DNA Dephos & Ligation Kit MilliporeSigma …fragments; dna ligase; ligases Storage: −20°C UNSPSC Code: 12352200 Components: rAPid Alkaline Phosphatase Buffer 10x concentrated; rAPid Alkaline Phosphatase 1 U/μl; DNA Dilution Buffer 5x concentrated; T4 DNA Ligation Buffer 2x concentrated; T4 DNA Ligase 5 U/μl … Compare this item peter thomsen wilster